Kemampuan Rhamnosidase Dari Isolat Kapang Untuk Hidrolisis Naringin Jeruk Siam
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Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian
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Rasa pahit pada olahan buah jeruk seperti jus dapat mengurangi preferensi konsumen. Saat ini enzim yang dapat mengurangi rasa pahit pada jeruk masih sulit diperoleh secara komersial. Tujuan penelitian ini adalah mengisolasi, memproduksi kapang dan menguji aktivitas enzim rhamnosidase yang dihasilkan. Isolasi kapang dilakukan terhadap sampel kulit, pulp dan jus jeruk Siam yang telah rusak. Seleksi dilakukan dengan metode plating menggunakan dua media yaitu RB dan G19. Ada lima isolat kapang penghasil enzim rhamnosidase yang berhasil diidentifikasi yakni: Penicillium brefeldianum, Penicillium restrictum, Aspergillus niger Rha-ase B, Aspergillus niger Rha-ase F dan Aspergillus niger Rha-ase H. Aktivitas spesifik enzim rhamnosidase maksimum dari kelima isolat kapang tersebut berturut-turut adalah: 189,34 U/mg protein, kultivikasi hari ke-7; 186,82 U/mg protein, kultivikasi hari ke-5; 456,18 U/mg protein, kultivikasi hari ke-6; 159,38 U/mg protein, kultivikasi hari ke-6 dan 386,63 U/mg protein, kultivikasi hari ke-2. Filtrat enzim rhamnosidase dari kapang A.niger Rha- ase H mempunyai kemampuan terbesar menurunkan kadar naringin pada larutan naringin konsentrasi 1000 ?g/ml dengan waktu kontak tiga jam yaitu sebesar 40,97 %.Effectivity of Rhamnosidase From Fungi Isolate on Hydrolisis of Naringin From Siam CitrusBitterness at many Siam citrus products like juice will decrease of cunsurner preferences. Now, enzyme that is able to reduce bitterness is difficult to be found commercially. The objectives of research were to isolate of mold, to produce and to know enzyme rhamnosidase activity from mold isolate. Isolation of mould was conducted at sample from skin of Siam, citrus juice and pulp which have been destroyed. Selection was conducted by plating method used two media that were RB and G 19. There were 5 identified isolates mould that produce rhamnosidase enzyme, namely: Penicillium brefeldianum, Penicillium restrictum, Aspergillus niger Rha-ase B, Aspergillus niger Rha-use F and Aspergillus niger Rha-ase H. %. The maximum spesific activity of rharnnosidase enzyme for 5 isolates as follows: 189.34 U I protein mg for 7th day cultivation; 186.82 U I protein mg for 5th day cultivation; 456.18 U I protein mg for 6th day cultivation; 159.38 U I protein rug for 6th day cultivation and 386.63 U I protein mg for 2nd day cultivation. To 3h contact at room temperature, rharnnosidase enzyme crude from A.n.iger Rha-ase H the biggest ability degrade 1000 1 g/ml naringin concentration that is equal to 40.97%.
Keywords
jeruk siam; kapang perusak jeruk; rhamnosidase; naringin; siam citrus; citrus mold spoiled.