The Effect of Coating Application Using Chitosan Enzymatic Depolymerization on Anthracnose Disease Suppression in Mango (Mangifera indica L.) cv. ’Arumanis’
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Date
2022-01-19
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Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
Abstract
Abstract. Mango cv. ’Arumanis’ is more prone to postharvest disease. An alternative anthracnose disease control is necessary to reduce use of synthetic pesticides. The research was aimed to develop bioformulation against Colletotrichum spp. Seventeen mango phyllosphere isolates belonging to Gram-positive (7 isolates) and Gram-negative (10 isolates) bacteria were obtained from Bogor and Pasuruan. The FAM 1 isolate produced the highest chitinase (14.7±1.11 ppm). Chitosan (CS) depolymerized with chitinase (D3K, BK2P of Bogor isolates) and mixed with tripolyphosphate (TPP) at a
concentration ratio of 5:1 (v/v) could inhibit the growth of Colletotrichum spp. compared with control under in vitro test, however, the level of inhibition was lower (<50%). Further formulations of CS (depolymerized with chitinase of Pasuruan isolates (EGM 1, FAM 1, and BMH) mixed with tripolyphosphate (TPP) using concentration ratio of 5:2 and
5:3 (v/v) was effective to suppress the fungal growth colonies in vitro. The application with a formula CS (FAM 1 isolate) and TPP mixture at ratio 5:2 showed as the highest anthracnose disease suppression in vivo. Candidate formulation to reduce Colletotrichum spp. infections have been obtained in the laboratory. However, in vivo test of various mango cultivars in the field still needs to be done.
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In Vitro, growth, Disease