Isolasi dan Identifikasi Molekuler Khamir yang Berkemampuan Memfermentasi Xilosa untuk Produksi Bioetanol Generasi Kedua
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KOMISI NASIONAL SUMBER DAYA GENETIK
Xylose is the second most abundant sugar after glucose from the hydrolysis of lignocellulosic agricultural waste. The application of microorganisms that ferment xylose and glucose is the main key for the production of second generation bioethanol. Yeasts are commonly used in the production of bioethanol only use glucose. This study aims to isolate and screen yeasts capable of fermenting xylose and glucose from fermented foods, and to identify them molecularly. Isolation of yeast using the spread method on YPXA (Yeast Potato Xylose Agar) and YPGA (Yeast Potato Glucose Agar) media. Yeasts that grow were evaluated its ability to produce ethanol using YPX and YPG liquid, whereas the molecular identification of yeasts were done with using ITS region sequence and PCR-RAPD analysis. Fermentation substrates and products were measured using HPLC. There were 24 isolates from four types of fermented foods using YPXA media and 22 isolates using YPGA media. KBKTI 10.5.1 isolate produced the highest ethanol yield from xylose fermentation, which was 16.84% per four days. TSX2 10-4.1.5 isolate was able to consume 12.72 g/L of xylose per four days with the product ethanol of 0.27 g/L. The best isolate to ferment glucose was DBY1 with 44.09% ethanol yield in four days fermentation. Yeasts isolates that ferment xylose are known to be Candida tropicalis, Cyberlindnera jadini, Wickerhamomyces anomalus, while the best species that ferment glucose is Saccharomyces cerevisiae and Pichia anomala. The yeast obtained has the potential for bioethanol production using lignocellulosic hydrolyzate.
Bioethanol, biofuel, lignocellulosic, yeast