Seminar Nasional Komisi Nasional Sumber Daya Genetik


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    Perbandingan Morfometrik Ayam Cemani Berdasarkan Perbedaan Tempat Konservasi
    (KOMISI NASIONAL SUMBER DAYA GENETIK, 2021-09-15) Kostaman ...[at al], Tatan; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    Cemani chicken is one of the genetic resources of native chickens from Temanggung Regency, Central Java which has the potential to be developed into superior chickens in Indonesia. Cemani chickens have wide spread and developed outside their natural habitat (in-situ). Thus, the purpose of this study was to compare and study the differentiating variables of chickens based on body sizes seen from their conservation sites. Morphometric of body measurements used as the basis for characterization were body length, chest length, chest width, chest circumference, back length, and shank length. The measurement of chicken body size variables was carried out on Cemani chickens in their natural habitat (in-situ) and ex-situ, which were kept in the Balitnak cage. The materials used were 22 Cemani chickens (14 hens and 8 roosters) from in-situ conservation and 36 Cemani chickens (11 hens and 25 roosters) from ex-situ conservation. The collected data were analyzed using the Independent Sample T-Test analysis. Differences in conservation sites showed significant results for the hen Cemani chicken body size variables (P<0.05), while the rooster Cemani chickens differed only for body length variables (P<0.05). Therefore, rooster Cemani chickens are relatively more uniform than hen chickens based on their conservation area.
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    Efektivitas Parasitoid Anisopteromalus calandrae (Howard, 1881) (Hymenoptera: Pteromalidae) sebagai Agen Biokontrol terhadap Sitophilus oryzae pada Media Jagung
    (KOMISI NASIONAL SUMBER DAYA GENETIK, 2021-09-15) Herlina, Lina; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    Sitophilus oryzae (Linnaeus) is an important pest of stored products serealia. Various control techniques have been made to control this pest, including use of parasitoids as natural enemies. The research of potential of Anisopteromalus calandrae (Howard 1881) as biocontrol agents of pests has been conducted at Insect Specimen Collection Lab in BB Biogen. Parasitoids were identified its morphological characters, and its ability in suppressing the population of S. oryzae was observed using 3 level of parasitoid densities i.e : inoculation of 0 insects, 8 insects, and 12 insects. The results showed that the inoculation of A. calandrae significantly influenced the number of S oryzae emerged, the percentage of intact seeds, and percentage of seed damage after 3 months of storage. The mean of emerging imago S. oryzae on the inoculation of 0, 8 and 12 parasitoids were 192.75 +47.26, 111.75 +29.61 and 48.00 +46.03 respectively. Mean intact seeds in the three treatments respectively were 102.88 + 6.25, 40.75 + 58.08, and 73.75 + 1.39. Mean seed damaged in the three treatments were 106.50 + 11.09, 66.00 + 11.58, and 34.50 +37.35. While mean of frassa in three treatment were 0.55 + 0.44, 0,39 + 0,11, and 0,19 + 0,17 but not sigificantly influenced by the treatment. Overall the experiment results showed that A. calandrae is potential as biocontrol agent to suppress population of S oryzae in maize.
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    Inisiasi dan Multiplikasi Tunas Rumput Gajah (Pennisetum purpureum) secara In Vitro pada Konsentrasi IBA Berbeda
    (KOMISI NASIONAL SUMBER DAYA GENETIK, 2021-09-15) Husni ...[at al], Ali; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    The objective of this research was to find out the effect of IBA concentration on the initiation and multiplication of elephant grass shoot explant through in vitro. The research was conducted from December 2019 to May 2020 at the Tissue Culture Laboratory, ICABIOGRAD. The research used one factor completely randomized design. The treatment in the initiation stages were used the different IBA concentrations (ppm), namely A0 (0), A1 (0.01), A2 (0.1), and A3 (1). In the multiplication stage there were B1 (1), B2 (2), and B3 (3). The result of this research indicates that there is no significant effect of any response design at the initiation stage. On the other hand, the result of the average value of explant growth showed that the use of IBA 1 ppm (A1) was more dominant (number of shoot, leave, and root) than the other concentrations. Then A2 treatment (IBA 0.1 ppm) was more dominant in the response to leaf length that was 7.51 cm and explant length that was 8.55 cm. In the multiplication stage, there was growth in the average number of shoots (1.8) shoots at the end of the multiplication stage. After that, in response to the number of leaves, there was also growth in the average number of leaves (3.93) at the final stage of multiplication. Moreover, there was growth in the average value of the number of roots for each stage with an average value of 3.23 roots in the final stage of multiplication.
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    Pengaruh Modifikasi Pakan Formula terhadap Aspek Biologi Ngengat Lilin Galleria mellonella (L.) (Lepidoptera: Pyralidae)
    (KOMISI NASIONAL SUMBER DAYA GENETIK, 2021-09-15) Rahmawati ...[at al], Vindri; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    The wax moth Galleria mellonella, a pest on honey comb, is also can be used as experimental insect to study biocontrol and as feed for birds. The aim of this research is to investigate the biological aspects of the wax moth on five formula diets to find a substitution of the expensive natural diets. Treatments consisted of five different diet formulas (A, B, C, D, and E) and natural honey comb as control. The result showed that the average life cycle on all formula diets ranged from 35.9 to 84.9 days, shorter than that on honey comb. Fecundity of female on diet formula reached 894-4565 eggs with fertility rate > 90%. The eggs hatched after 4-14 days of incubation period. The duration of larval and prepupal stages was shorter on formula diets than that on honey comb. Conversely the longevity on formula diets was greater than on natural diets. Adult longevity varied from 1 to 27 days, and male adults lived longer (11.8 days) than females (6.2 days). In general, all formula diets tested (except C) showed better effects than honey comb for mass rearing of G. mellonella. Diet formula E considered as the most suitable diet for mass rearing G. mellonella.
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    Karakterisasi Molekuler Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) Menggunakan Sekuen DNA Polimerase
    (KOMISI NASIONAL SUMBER DAYA GENETIK, 2021-09-15) Yusuf ...[at al], Sela; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    Corn borer (Helicoverpa armigera) is one of the most important pests that threaten corn production. H. armigera attacks cause corn production to decrease up to 80%. The use of the biological agent Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) is one of many ways to control the pest’s population along with the principles of Integrated Pest Management (IPM). This study aims to obtain the molecular characteristics of H. armigera NPV . NPV isolates used obtained from a collection of Insect Pathology Laboratory that were propagated in H. armigera larvae that were reared in the laboratory. Isolated DNA was amplified using the PCR method using forward primer (5'-GAT CGT TGA CCG ACG TCA ATA -3 ') and reverse primer (5'-CGA TGA CAC GTA GCC AGT AAA -3'), then homology and phylogenetics tests are carried out to analyze the relationship of HearNPV Bogor isolate with NPV isolates from other countries that have been registered on GeneBank. Based on the analysis of the nucleotide and amino acid sequences of DNA polymerase, the Bogor HearNPV isolate had a genetic relationship with NPV that infect H. armigera from Spain and India, H. assulta from China and H. zea from Brazil. Based on phylogenetic analysis the Bogor HearNPV is in the same group as NPV of H. armigera from Spain and India, H. assulta from China and H. zea from Brazil.