Seminar Nasional Komisi Nasional Sumber Daya Genetik

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Browsing Seminar Nasional Komisi Nasional Sumber Daya Genetik by Subject "Anthracnose, chilli pepper, molecular marker"

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    Identifikasi 27 Varietas Cabai Menggunakan Beberapa Jenis Marka Molekuler dan Asosiasinya dengan Ketahanan Antraknosa
    (KOMISI NASIONAL SUMBER DAYA GENETIK, 2021-09-15) Terryana ...[at al], Rerenstradika Tizar; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    Chilli is a strategic horticultural commodity in Indonesia with high economic value and needs improvement for anthracnose resistance. Molecular markers availability has increased the development of DNA fingerprinting technology for various objectives, i.e. genetic diversity, varietal identification, and assisting selection stage in plant breeding. This study aimed to identify commercial varieties of chilli in Indonesia using several types of PCR-based molecular markers and their association with anthracnose resistance. A total of 27 chilli varieties consisting of into two species groups (Capsicum annuum L. and C. frutescent L.) were genotyped using 12 molecular markers (8 SNAP (single nucleotide amplified polymorphisms), 2 RAPD (random amplified polymorphic DNA), 1 INDEL (insertions-deletions), and 1 SSR (simple sequence repeats)). Varietal resistance to anthracnose was also tested in vitro using Colletotrichum capsici isolate. The results showed an average polymorphism information content (PIC) value of 0.24 (0.07-0.71). Based on phylogenetic analysis, 27 chilli varieties were divided into two main groups with different genetic similarity coefficients depending on the type of molecular markers. Three sets of molecular markers, which are 8 SNAP markers, a combination of 4 markers (2 RAPD, 1 INDEL, and 1 SSR), and all of the molecular markers used, can clearly distinguish chilli varieties but at different cut points, 0.78, 0, 60 and 0.67, respectively. The combined 4 types of molecular markers showed the highest genetic differentiation value for chilli varieties used. Importantly, OPE18 was significantly associated with resistance to anthracnose, which is promising to assist the selection process in chilli breeding after further assay.