Genetic Variation of Adan, a Krayan Local Rice Mutant, Using Microsatellite Markers
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Date
2022-01-19
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Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
Abstract
Abstract. One of the famous local rice in the border region of North Kalimantan Province with Malaysia is Adan rice. However, the long growth duration of Adan rice (around six months) means that local farmers can only plant this rice once a year. Efforts to shorten the life of Adan rice have been carried out using gamma-ray radiation, and resulted an
early heading mutant lines with four-month old possessing physical characteristics similar to Adan rice. This study aimed to evaluate genetic variaton on selected Adan rice mutants using SSR markers. This research was conducted at the Molecular Biology Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and
Development, from February to July 2015. A total of eight selected short-duration mutants and Adan cultivar (its wild type) were genotyped using seventy one SSR markers located on all rice chromosomes. Genetic similarity among tested samples was determined using the Dice coefficient, followed by grouping the samples using the UPGMA method
facilitated in NTSYSpc-2.02 software. The seventy one SSR markers produced 19 bands, 110 alleles with the PIC value ranged from 0.000.97. The dendrogram grouped the mutant lines into two groups at a similarity level of 65%. The first group contained Adan rice, A28, A25, and A27 rice mutant lines, while the second ones consisted of A51, A88, A93.1, A93.2, and A50 rice mutant lines. The group I can be used as candidates for mutant development at Krayan District, where Adan rice is usually planted. This research also showed that SSR markers can be used to identify the genomic changes in rice mutants.
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Keywords
Rice, genetic, Microsatellite Markers