Seed Genetic Purity Assessment of Hybrid Maize Using Microsatellite Markers (SSR)
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Date
2013
Journal Title
Journal ISSN
Volume Title
Publisher
Badan Penelitian dan Pengembangan Pertanian
Abstract
The development of hybrid varieties should be supported by the availability of quality seeds. Genetic purity is one of the quality criteria required for successful seed production of hybrid maize. In producing hybrid seeds are often contaminated by crosses pollen from another variety or the occurrence of selfing. The objective of this study was 1) to get microsatellite markers (SSR) specific for male and female parents, 2) to get a percentage of the genetic purity of hybrid maize seeds cv.Bima-3 and Bima-4. The study was conducted at the Laboratory Indonesian Center for Agricultural Biotechnology and Genetic Resources Bogor, from April until December 2011. Parental lines used were to production hybrid cv. Bima-3 and Bima-4. Hybrid maize varieties and their parental lines seed used were derived from Indonesian Cereals Research Institute (BALITSEREAL) Maros and markers SSR selected for parental lines were phi109275, phi96100, phi374118, phi328175, and phi072. The assessment of genetic purity used two hybrid varieties namely cv.Bima-3 and Bima-4, and used specific markers resulted from identification for both parental hybrid. Fourty samples individual plants of each hybrid maize variety were tested. From 5 markers tested, three of them produced polymorphic bands namely phi96100, phi328175 and phi072. The phi96100 was specific to parental cv.Bima-4, phi072 was specific for parental cv.Bima-3. While phi328175 was specific markers to parental of both hybrids maize were tested. The test of the genetic purity showed that 80% of the cv.Bima-4 and 97.5% cv.Bima-3 hybrid seeds genetically pure. The results of study are expected to be useful in the verification and seed purity test varieties of hybrid maize faster and more accurate