Keragaman Genetik 30 Aksesi Kedelai Introduksi Berdasarkan Marka Single Nucleotide Amplified Polymorphism (SNAP)
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Date
2021-09-15
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KOMISI NASIONAL SUMBER DAYA GENETIK
Abstract
Soybean breeding programs aim to support soybean self-sufficiency by increasing seed productivity in conjunction with other important characters. This requires wide genetic diversity of germplasms collection. Introduction varieties is one of the way to increase genetic diversity of soybean germplasms as a basic principle in breeding program. Nowadays, molecular markers have been widely applied in genetic diversity analysis which is important in assisting soybean pre-breeding process. The purpose of this study was to analyze the genetic diversity of 30 introduced soybean accessions by using ten SNAP markers. Genomic DNA from 30 introduced accessions with three national varieties i.e Wilis, Anjasmoro, and Tanggamus were amplified using ten pairs of newly design SNAP primers. The results showed that all of primers were able to produce clearly visible amplicon bands and validated their SNPs in the three national varieties according to in silico analysis. The scoring result showed as many as 69% alleles, possessed by introduced accessions, were alternative alleles that similar to Wilis, Anjasmoro, and Tanggamus. The phylogenetic analysis showed that there was a grouping of introduced accessions in two main clusters based on the SNP alleles. There were two accessions from Japan, namely Shirojisi and 3447, showed grouping in the same sub-cluster with the national varieties. The SNAP markers showed successful validation to detect polymorphisms based on nucleotide differences without the need for sequencing and are expected to be able to support the acceleration of soybean breeding programs, especially in the assembly of new improved varieties with required targetted characters.
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Genetic diversity, molecular marker, soybean introduction, SNP, SNAP