The Application of Gamma Ray Irradiation to Increase Triterpenoid Compounds in Embryogenic Calli of Centella asiatica L. Urban
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Date
2022-01-19
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Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
Abstract
Abstract. Centella asiatica L. Urban has bioactive compounds from triterpenoid group. Raw materials that are not standardized are one of the obstacles in supplying the needs of the pharmaceutical industry. The in vitro culture technology may solve this problem. The purpose of this study was to determine the effect of gamma ray irradiation on growth and triterpenoid content of embryogenic calli of C. asiatica. The three accessions tested were CASI 016, CASI 002, and released varieties Castina 1. Gamma ray irradiation was carried out at doses of 0, 1, 2, 3, 4, and 5 Krad. The results showed that the in vitro response of each accession was genotype dependent. Castina 1 contained higher triterpenoid compounds than the other accessions. However, the gamma ray irradiation treatment up to 5 Krad has not been able to increase the content of these compounds. The treatment could only increase the content of asiaticoside and asiatic acid of accession CASI 016 at the dose of 5 Krad. There was an increase of asiaticoside and madecasoside content
of accession CASI 002. It is expected that the increase of gamma ray irradiation dose will increase the triterpenoid compounds content in embryogenic calli of C. asiatica.
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Keywords
In Vitro, embryogenic, Irradiation