Keragaman Genetik 82 Aksesi Padi Liar (Oryza spp.) Menggunakan Marka Mikrosatelit dan Sequence Tagged Site (STS)
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Date
2021-09-15
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KOMISI NASIONAL SUMBER DAYA GENETIK
Abstract
Wild rice (Oryza spp.) is a relative of cultivated rice (Oryza sativa) with specific morphological characters and has a genetic potential for rice breeding. Genetic diversity has an important role in plant breeding because the information and understanding of it can help the success of plant breeding programs. The purpose of this study was to identify the character of each wild rice accession using microsatellite markers and Sequence Tagged Sites (STS) and identify the relationship between wild rice accessions using microsatellite markers and Sequence Tagged Sites (STS). Microsatellite or Simple Sequence Repeat (SSR) is the most widely used marker for genetic diversity analysis, especially to determine the level of heterozygosity. Leaves sample of wild rice plants were taken and collected for DNA isolation. Wild rice DNA was isolated using of Doyle and Doyle method (1990). Wild rice DNA was amplified using 3 SSR markers. DNA bands were scored as binary. Binary data from SSR markers then were analyzed for calculating Polymorphism Information Content (PIC), number of alleles, allele frequency, gene diversity, and heterozygosity using PowerMaker software. Relatedness among accessions were analyzed using NTSYS 2.1. Principal Coordinates Analysis (PCoA) was analyzed using the XLSTAT Trial 2020. The results from the genetic diversity analysis of 82 wild rice accessions showed that the genetic similarity coefficient was 0.44 based on 3 SSR. Three main clusters were formed in this study. The first cluster consisted of 69 genotypes, the second cluster consisted of 8 genotypes, and the third cluster consisted of 5 genotypes. All SSR and STS markers used are very informative because it has a PIC value > 0.5, thus estimating genetic diversity among wild rice genotypes.
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Genetic diversity, PIC, wild rice