Response of Gamma Ray Irradiation Derived-Cultures of Three Sugarcane Varieties to Drought Stress Induced by Polyethylene Glycol
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Date
2022-01-19
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Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
Abstract
Abstract. Drought is one of limiting factor in sugarcane growth and productivity, therefore sugarcane which has high production in the dry season is needed. Somaclonal variation combined with in vitro selection could increase genetic variation, while those new variants can be selected in vitro. The purpose of this study was to evaluate the response of
somaclones derived from gamma ray irradiation combined with in vitro selection by using polyethylene glycol (PEG). Three new varieties of sugarcane (AAS Agribun, CMG Agribun, and AMS Agribun) were used as plant materials. Embryogenic calli were induced using 3 mg/l 2,4-D and 3g/l. casein hydrolysate followed by gamma ray irradiation at
doses of 10, 20, 30, 40, 50, and 60 Gy. After recovery step, calli were selected by using PEG 6000 10 and 15%. The results showed that AMS Agribun derived-cultures were growth faster to form callus, but they were most sensitive after treated with gamma ray irradiation. The highest percentage of callus regeneration were obtained from AAS Agribun mutants after selected with PEG. The highest number of mutant shoots tolerant to PEG were obtained from AAS Agribun and the lowest from CMG Agribun. The higher gamma ray irradiation dose more than 30 Gy and in vitro selection more than 10% inhibited the growth of cultures.
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Keywords
Sugarcane, growth, genetic