DNA Barcoding of Vatica bantamensis, a Critically Endangered Tree Endemic to Banten, Indonesia
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Date
2022-01-19
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Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
Abstract
Abstract. Vatica bantamensis is a member of the family Dipterocarpaceae endemic to Ujung Kulon National Parks in Banten Province, Indonesia. It is classified as a critically endangered species by IUCN Red List. Under this category, the species is considered to be facing an extremely high risk of extinction in the wild. The objectives of this study were to provide easy and accurate identification of V. bantamensis for the conservation and sustainable use of the species. In the present study, we conducted DNA barcoding of V. bantamensis using an rbcL marker. We used V. rassak as a comparison species since both species are morphologically closely related. A total of 606‒608 bp nucleotides have been successfully amplified and sequenced. The dendrogram constructed using the neighbor-joining method with Tamura 3-parameter showed that V. bantamensis used in this study were clustered together with V. rassak. These two species were separated clearly against other Vatica species and two outgroup species, namely Dryobalanops lanceolata and Shorea pinanga, obtained from the NCBI database. The rbcL sequence can discriminate V. bantamensis from V. rassak in six different sites (136, 507, 522, 565, 566, and 567). This study demonstrated that the rbcL sequence could authenticate the
samples of V. bantamensis and support the correct identification of this endangered plant species.
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Keywords
Vatica bantamensis, DNA, Banten