The Effect of FeSO4 Concentration on the Callus Growth of Two Chili (Capsicum annuum L.) Varieties
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Date
2022-01-19
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Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
Abstract
Abstract. In vitro selection is a method that can produce varieties that are tolerant to abiotic stresses such as Fe poisoning. FeSO4 is a compound that can be used as an in vitro selection agent for resistance to Fe toxicity. Each genotype has a different response to FeSO4. For this reason, it is necessary to determine the optimal concentration for
each genotype to be selected so that the selection results are more accurate. This study aimed to observe the response of callus growth to Fe toxicity and to obtain the lethal concentration of 50 (LC50) FeSO4 value for callus of two chili varieties Prima Agrihorti and Rabani Agrihorti. This study used a complete factorial design with the first factor was
variety and the second factor was the concentration of FeSO4 (0, 54, 108, 162, and 215 mg/l). The number of repetitions was 10 times. The observed parameters were the percentage of browning callus, callus diameter, and callus performance. To determine the LC50 value, the curve analysis program was used. The results showed the interaction between varieties and the concentration of FeSO4 has not significant effect, but when the FeSO4 concentration increase, the growth was inhibited, shown as smaller diameter and the callus turned to brown. The LC50 FeSO4 value in the variety Prima Agrihorti
was 130.341 mg/l, while that of Rabani Agrihorti was 115.511 mg/l. These values can be used for in vitro selection of callus of chili varieties Prima Agrihorti and Rabani Agrihorti.
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Chili, Growth, in vitro