Kemampuan Antagonis Bakteri Lipolitik asal Tanah terhadap Ganoderma
| dc.contributor.author | Sofiana ...[at al], Indah | |
| dc.contributor.other | Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian | en_US |
| dc.date.accessioned | 2021-12-23T07:08:39Z | |
| dc.date.available | 2021-12-23T07:08:39Z | |
| dc.date.issued | 2021-09-15 | |
| dc.description.abstract | This study aims to obtain lipolytic bacteria isolates and has antagonistic ability against Ganoderma. The Ganoderma isolates used were Biogen Culture Collection (BiogenCC). Bacterial isolates were isolated from the soil. Lipolytic test results obtained as many as 21 bacterial isolates capable of producing lipase enzymes. The lipolytic test was carried out qualitatively. The indicator of lipolytic activity was the formation of a clear zone around the colony on Tributyrin media for modification. The antagonist test was carried out using the dual culture method on PDA media. The results of the antagonist test against BiogenCC gan-1 showed 10 potential isolates, and against BiogenCC gan-2, 1 potential isolate was obtained. Based on the calculation of the percentage of antagonist test resistance, there was 1 bacterial isolate that gave the best results, including isolates E2.M3 (10-3) (77.78%) against BiogenCC gan-1, and B2.M2 (10-3)(59.4%) against BiogenCC gan-2. Antagonist test indicator is the formation of inhibition zones on PDA media. Molecular identification showed that BiogenCC gan-1 & BiogenCC gan-2 isolates were identified as Ganoderma boninense with sequence homology above 98%. Isolates E2.M3 (10-3) & B2.M2 (10-3) were identified as Lysinibacillus pakistanensis, with sequence homology above 99%. | en_US |
| dc.identifier.isbn | 978-9798-3930-7-5 | |
| dc.identifier.uri | https://repository.pertanian.go.id/handle/123456789/14479 | |
| dc.language.iso | id | en_US |
| dc.publisher | KOMISI NASIONAL SUMBER DAYA GENETIK | en_US |
| dc.subject | Antagonist, bacteria, Ganoderma, lipolytic | en_US |
| dc.title | Kemampuan Antagonis Bakteri Lipolitik asal Tanah terhadap Ganoderma | en_US |
| dc.type | Book | en_US |