Deteksi Gen Tet pada Tanaman Kentang PRG Katahdin Event SP951 dan Hasil Persilangannya dengan PCR
No Thumbnail Available
Date
2021-09-15
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
KOMISI NASIONAL SUMBER DAYA GENETIK
Abstract
The development of transgenic potato carrying RB gene was conducted through Agrobacterium tumefaciens on Katahdin and have produced transgenic Katahdin event SP951. Hybridization between Atlantic and Granola with transgenic Katahdin event SP951 produced six selected transgenic clones containing RB gene that were resistant to Phytophthora infestans in several confined field trials. Before being released to the market, a transgenic plant should be declared safe for the environment, food and feed. One study of environmental safety is the analysis of environmental risk using laboratory data related to inserted gene. The objective of the research is to analyze the content of Tet gene on the pCLD04541 plasmid that carries RB gene in transgenic Katahdin event SP951 and six selected transgenic hybrid clones using PCR. The results of the analysis showed that six selected transgenic hybrid clones were not indicated Right Border (RB) fragment size 539 bp. Clone 27, 66 and transgenic Katahdin event SP951 (79) produced Left Border (LB) fragment size 246 bp. Six selected transgenic hybrid clones and transgenic Katahdin event SP951 were not produced Tet gene fragment size 452 bp. Six selected transgenic hybrid clones and transgenic Katahdin event SP951 should be safety because did not contain integrated antibiotic gene inside the plant genome.
Description
Keywords
Agrobacterium tumefaciens, environmental safety, plasmid backbone, Tet gene, transgenic potato