Mutasi Radiasi Kombinasi dengan Kultur In Vitro pada Kedelai Varietas Wilis, Grobogan dan Dering-1 untuk Meningkatkan Keragaman Genetik pada Mutan M2
No Thumbnail Available
Date
2021-09-15
Journal Title
Journal ISSN
Volume Title
Publisher
KOMISI NASIONAL SUMBER DAYA GENETIK
Abstract
Soybean (Glycine max (L.) Merr.) is one of the important commodities to be developed as a supporter of food and industrial security, but its development is experiencing problems due to its low productivity. To overcome this problem, breeding research is still needed to increase its variability as material for selection. The aim of this research is to irradiate young soybean embryos of Wilis, Grobogan, and Dering-1 varieties for obtaining putative mutants that have better characteristics. The research was carried out at the Cell and Tissue Biology Laboratory of ICABIOGRAD in 2017. The explants used were young soybean embryos of Wilis, Dering-1 and Grobogan varieties. Explants were treated with 4Gy gamma irradiation, then cultured on MS + BA (0.1 and 0.5 mg/l) and Kinetin (0.1 and 0.5 mg/l) media. The resulting plantlets were acclimatized to produce M1 seeds, then the resulting seeds were replanted to produce an M2 mutant population. The results obtained show that the given gamma ray radiation caused inhibition of shoot growth and root formation so that not many plantlets were obtained as well as plantlets that were successfully acclimatized were very limited. The observations result of plant growth (M2) in the greenhouse reveal that there were differences in the yield components of each mutant produced, i.e. plant height, as well as number of branches and pods. From the results obtained, it can be concluded that the radiation dose given was able to produce variations in the yield components, and obtained putative mutant having more pods than the parents.
Description
Keywords
Gamma ray irradiation, mutation, soybean