Sucrose and Putrescine Increased Callus Induction in Tomato Anther Culture
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Date
2022-01-19
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Publisher
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
Abstract
Abstract. Androgenesis induction through anther culture technique is one of supplementary tools to accelerate selection program in classical breeding. Doubled-haploid (DH) derived-anther culture plants can be readily selected due to their
high homozygosity. In this study, the effect of putrescine and various concentrations of sucrose in callus induction medium were investigated in anther culture of a tomato. Flower buds (4–6 mm) of a hybrid tomato cv. Permata containing anthers (1.5–2.5 mm) were used as anther donor material. The anthers were cultured on DBM1 callus induction medium containing sucrose at a concentration of 2.0%, 4.0%, 6.0%, and 8.0% with and without putrescine. The results showed that the onset of callus formation was affected by the concentration of sucrose, while the duration of callus formation was affected by the addition of putrescine into callus induction media. However, the number of calluses
formed differed significantly with the concentration of sucrose and putrescine. The best medium to prolong anther viability and increase callus induction was DBM I + kinetin 5 mg/l + NAA 2 mg/l + 10-3 M putrescine + 2% sucrose.
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Keywords
Tomato, breeding, culture technique