Characteristic of Indigenous Leuconostoc mesenteroides EN 17-11 Protease and Its Stability during Storage at Cold and Freezing Temperatures
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Date
2022-01-19
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Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
Abstract
Abstract. Protease as protein hydrolyzer is an imported enzyme used in the food and pharmaceutical industries in Indonesia. To reduce imports, indigenous lactic acid bacteria can be used as a source of protease. This study aimed to determine the characteristics of indigenous Leuconostoc mesenteroides EN 17-11 protease and its stability during storage
at cold and freezing temperatures. Protease activity was detected using the modified Horikoshi method. The protease stability after storage was tested at 0, 7, 14, 21, and 28 days. Relative protease activity with a value ≥50% indicates protease activity in stable conditions. All treatments were statistically analyzed using ANOVA. The results showed that the optimum protease activity of L. mesenteroides EN 17-11 was reached at pH 6.0 with activity of 0.9970 U/ml and temperature of 40°C with activity of 1.1166 U/ml (p<0.05). The protease stability at incubation time of 60 min was reached at pH 5.0–7.5 with a relative activity of 55.29–100.00%, and a temperature of 20–50°C with a relative activity of
51.92–100.00% (p<0.05). The protease stability at storage for 28 days with a relative activity of ≥50% at freezing temperature was higher than at cold temperature. The highest protease stability was reached at freezing temperature for 7 days with a relative activity of 77.31% (p<0.05). It was concluded that the protease stability of indigenous L. mesenteroides EN 17-11 was better at freezing than at cold temperature.
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Keywords
Enzyme, hydrolyzer, bacteria