Analisis Keragaman Genetik Aksesi Ubi Jalar Lokal Menggunakan Marka Simple Sequence Repeat (SSR)
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Date
2021-09-15
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KOMISI NASIONAL SUMBER DAYA GENETIK
Abstract
Indonesia is the second center of origin after South America for sweet potato (Ipomoea batatas (L.) Lamb.) genetic resources, with the highest diversity found in Papua. This commodity is widely cultivated and uses both for food and industrial raw materials in various regions of Indonesia. The objective of this research is to study genetic diversity of sweet potato accessions collected from various areas in Indonesia. A total of 90 accessions of local sweet potatoes collected from Java, Nusa Tenggara, Sulawesi and Papua were used in this study. DNA was extracted and isolated for further molecular analysis using 18 SSR markers. The results showed that of the 18 SSR markers used, 13 markers (72%) were highly informative (PIC>0.73). From the results of the analysis, the average value of the major allele frequency, the number of alleles, gene diversity, heterozygosity and polymorphic information content/PIC were 0.32, 13.7, 0.79, 0.38, and 0.77, respectively. The IBSSR19 marker is known to be the most productive and informative marker because it is able to display the highest number of alleles (27 alleles), the highest gene diversity (0.92) as well as the highest PIC value (0.91). The result of cluster analysis showed that 90 accessions of sweet potato were separated into three groups, each consisting of 32, 46 and 12 accessions. The second group is further separated into two sub-groups, each consists of 20 and 26 accessions. This grouping does not reflect with the provenance of the accessions.
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Keywords
Genetic diversity analysis, SSR markers, sweet potato