Regenerasi Tanaman melalui Embriogenesis Somatik dan Beberapa Gen yang Mengendalikannya
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Date
2002
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Balai Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian
Abstract
Plant Regeneration trough Somatic Embryogenesis and Some Gene Expression.
Ragapadmi Purnamaningsih. The agriculture system need to be enhanced. In order that it is needed the qualified and uniform seedlings in plenty quantity. However it is very difficult to get that from conventional method. By using tissue culture method, seedling can be multiplied. The advantages of this method are (1) seedling can be obtained in plenty quantity, (2) seedling can be obtained in short time relatively, and (3) the seedlings have the same character with the mother plant. Multiplication of explant by using embryogenesis somatic is the one technique that can be applied. With this technique explant can be induced to form somatic seedling from several specific stages: embryogenic callus induction, maturation, germination, and hardening. All of the stages are depend on kind of explant and the composition of nutrition in the culture media (carbohydrate sources, kind, and concentration of plant growth regulator, etc.).
Embryogenesis somatic process is regulated by several genes CHB3, CHB4, CHB5, and CHB6. The expression of these genes determine the stage of embryo development untill somatic seedlings are formed.
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Tissue culture, embryogenesis, gene