In Vitro Organogenesis of Soybean and Agronomic Characterization of Obtained Plants

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Central Research Institute for Food Crops
Organogenesis of soybean through shoot bud induction was done using cotyledonry nodes of soybean cultivars Sicinang and Sriyono as sources of explants. A Murashige-Skoog (MS) medium supplemented with thidiazuran increasing from 0.005 to 0.05 mg/l plus NAA or 2,4-D 0.01 mg/l was used in the study. The shoots obtained were subcultured and the original cotyledonary nodes were cut off after subculturing the shoots. Growths and productions of the Fi and F2 in vitro derived plants were recorded. The results indicated that increasing concentration of thidiazuron from 0.005 to 0.05 mgfl and the presence of either NAA or 2,4-D 0.01 mg/l in the medium were beneficial to shoot regeneration. During the induction period, the average number of shoots obtained were 5.6 from cultivar Sicinang and 5.2 from cultivar Sriyono. The shoots were then cut off and subcultured into a fresh medium containing 0.01 mg/l thidiazuron. in the first subcultures, the numbers of shoots ranged from 2.8-6.2 shoots per explants, while the cotyledonary node produced 21-25 shoots. After the third subcultures, the cotyledons dried out and no further buds were formed. Plants derived from in vitro cultures of both subcultures I and II were shorter than the original seed derived plants. The seed derived plants were also mature earlier and more tolerant to shading of up to 70% shading. The plants obtained from in vitro subculture seem to be more suitable to be grown intercropped with other plants
In vitro, organogenesis, Glycine max