Browsing by Author "Suryadi ...[at al], Yadi"

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    Bioprospeksi Berbasis Sumber Daya Genetik Mikroba Untuk Pengelolaan Penyakit Tanaman
    (IAARD Press, 2018-11) Suryadi ...[at al], Yadi; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
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    The Effect of Coating Application Using Chitosan Enzymatic Depolymerization on Anthracnose Disease Suppression in Mango (Mangifera indica L.) cv. ’Arumanis’
    (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, 2022-01-19) Suryadi ...[at al], Yadi; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
    Abstract. Mango cv. ’Arumanis’ is more prone to postharvest disease. An alternative anthracnose disease control is necessary to reduce use of synthetic pesticides. The research was aimed to develop bioformulation against Colletotrichum spp. Seventeen mango phyllosphere isolates belonging to Gram-positive (7 isolates) and Gram-negative (10 isolates) bacteria were obtained from Bogor and Pasuruan. The FAM 1 isolate produced the highest chitinase (14.7±1.11 ppm). Chitosan (CS) depolymerized with chitinase (D3K, BK2P of Bogor isolates) and mixed with tripolyphosphate (TPP) at a concentration ratio of 5:1 (v/v) could inhibit the growth of Colletotrichum spp. compared with control under in vitro test, however, the level of inhibition was lower (<50%). Further formulations of CS (depolymerized with chitinase of Pasuruan isolates (EGM 1, FAM 1, and BMH) mixed with tripolyphosphate (TPP) using concentration ratio of 5:2 and 5:3 (v/v) was effective to suppress the fungal growth colonies in vitro. The application with a formula CS (FAM 1 isolate) and TPP mixture at ratio 5:2 showed as the highest anthracnose disease suppression in vivo. Candidate formulation to reduce Colletotrichum spp. infections have been obtained in the laboratory. However, in vivo test of various mango cultivars in the field still needs to be done.
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    Kemajuan Teknik Deteksi dan Identifikasi Pseudomonas solanacearum
    (Balai Penelitian Bioteknologi Tanaman Pangan, 1996) Suryadi ...[at al], Yadi; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    Y. Suryadi and M. Machmud. 1996. Progress on Detection and Identification of Pseudo monas solanacearum. Buletin AgroB/o 1(1): 11-17. Bacterial wilt caused by P. Solanacearum is a serious disease on various crops. The difficulties in the disease control is due to genetic variability of the pathogen. In the past few years, detection and identification of P. Solanacearum which was based on host ranges and physiological/ biochemical characteristics, resulted in five races and five biovars. The classification was not satisfactorily accepted. It is necessary to develop early, rapid, and accurate disease detection techniques amenable for analysis of genetic variability of the pathogen. This paper overviewed progress on P. solanacearum detection based on physiological and biochemical characteristics, such as the use of Bactid, Biolog, fatty acid profiles, and protein profiles. In addition new approaches on molecular P. solanacearum detection through DNA fingerprinting i.e., RFLP, RAPD, ribotyping, substractive hybridization and PCR were also described. Future prospect on the application of these techniques in Indonesia is mentioned.
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    Respon Beberapa Genotipe Kacang Tanah terhadap Penyakit Layu Bakteri (Ralstonia solanacearum) di Rumah Kaca
    (Sekretariat Komisi Nasional Plasma Nutfah, 2009) Suryadi ...[at al], Yadi; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    Penelitian bertujuan untuk mempelajari tingkat ketahanan genotipe kacang tanah terhadap penyakit layu bakteri (Ralstonia solanacearum) di rumah kaca Kelompok Peneliti Biokimia BB-Biogen. Isolat R. solanacearum diperoleh dari tanaman kacang tanah yang menunjukkan gejala layu dari Desa Karadenan (Cibinong). Penyiapan dan penyediaan inokulum bakteri R. solanacearum menggunakan media SP (sukrose pepton). Tanaman kacang tanah diinokulasi dengan isolat R. solanacearum dengan kerapatan koloni 107 cfu/ml. Penelitian disusun menggunakan rancangan acak kelompok dengan tujuh perlakuan dan empat ulangan. Untuk pembanding tahan dan rentan masing-masing digunakan genotipe Tupai dan Kelinci. Hasil penelitian menunjukkan bahwa genotipe ICGV 88262, lokal Sindangbarang, PI 203395, ICG 10067, dan ICG 3400 tahan terhadap penyakit layu bakteri dengan nilai kerusakan <20%. Penyakit layu bakteri berpengaruh terhadap penurunan beberapa karakter fenotipik kacang tanah, di mana tanaman genotipe kacang tanah tahan PLB relatif lebih tinggi dibandingkan dengan genotipe Kelinci. Polong bernas Kelinci paling sedikit di antara semua genotipe uji.