Kemajuan Teknik Deteksi dan Identifikasi Pseudomonas solanacearum
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Date
1996
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Balai Penelitian Bioteknologi Tanaman Pangan
Abstract
Y. Suryadi and M. Machmud. 1996. Progress on Detection and Identification of Pseudo monas solanacearum. Buletin AgroB/o 1(1): 11-17. Bacterial wilt caused by P. Solanacearum is a serious disease on various crops. The difficulties in the disease control is due to genetic variability of the pathogen. In the past few years, detection and identification of P. Solanacearum which was based on host ranges and physiological/ biochemical characteristics, resulted in five races and five biovars. The classification was not satisfactorily accepted. It is necessary to develop early, rapid, and accurate disease detection techniques amenable for analysis of genetic variability of the pathogen. This paper overviewed progress on P. solanacearum detection based on physiological and biochemical characteristics, such as the use of Bactid, Biolog, fatty acid profiles, and protein profiles. In addition new approaches on molecular P. solanacearum detection through DNA fingerprinting i.e., RFLP, RAPD, ribotyping, substractive hybridization and PCR were also described. Future prospect on the application of these techniques in Indonesia is mentioned.
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Keywords
Pseudomonas solanacearum, detection, identification