Prosiding Seminar Nasional Sumber Daya Genetik dan Pemuliaan Tanaman

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Browsing Prosiding Seminar Nasional Sumber Daya Genetik dan Pemuliaan Tanaman by Author "Carsono ...[at al], Nono"

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    Identifikasi Gen Kegenjahan Padi Generasi F2 Hasil Persilangan Kultivar Ciapus X Kitaake Menggunakan Dua Marka SSR Serta Korelasinya Dengan Karakter Umur Keluar Malai
    (IAARD Press, 2012-12) Carsono ...[at al], Nono; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    Identification Of Early Maturing Gene in F2 Generation of Rice Derived from Cross between Cv. Ciapus x Kitaake by using Two Ssr Markers and its Correlation with Heading Date. Developing of early maturing rice is very important for increasing rice crop productivity in Indonesia. The use of molecular markers, such as SSR markers, in selecting desired genotype will be tremendously helpful. SSR markers are widely used since they are simple, economic, accurate, codominant and has high polymorphic level. The objective of current experiment was to obtain genotype with molecularly (SSR profile) as well as phenotypicaly characterized (early heading date). One hundred two plants of F2 generation of rice derived from cross between cv. Ciapus (high productivity) and Kitaake (earliest of heading date) were selected based on molecular and phenotypic markers of early flowering traits. Heading date was used as phenotypic marker, whereas molecular markers used were SSR markers of RM7601 (detecting Hd2) and RM19414 (detecting Hd3). PCR analysis was conducted at Lab of Plant Analysis and Biotechnology, meanwhile field experiment was performed at Ciparanje Experimental Station, Faculty of Agriculture, Universitas Padjadjaran. Results indicated that thirty-seven plants were selected on the basis of the presence of SSR markers. Genotypes # 136 and # 300 were the promising ones based on both molecular and phenotypic markers. However correlation coefficient were 0.012 for RM7601 and -0.142 for RM19414, respectively, indicating a weak relationship between molecular markers with phenotypic markers. This could be due to a large effect of environmental factors on heading date. Two plants (# 136 and 300) are highly recommended for future research in the development of early maturing rice lines.
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    Pola Pewarisan Karakter Umur Keluar Malai dan Seleksi Pada Generasi F2 Hasil Persilangan Padi Ciherang X Kitaake
    (IAARD Press, 2012-12) Carsono ...[at al], Nono; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    Pola pewarisan merupakan sumber informasi untuk seleksi, mekanisme pewarisan karakter, dan mengestimasi banyaknya gen pengendali. Penelitian ini bertujuan untuk mendapatkan pola pewarisan karakter umur keluar malai dari hasil persilangan antara Ciherang x Kitaake, dan untuk seleksi umur singkat keluar malai pada tanaman. Percobaan ini dilaksanakan sejak Maret 2011 sampai dengan Agustus 2011 di lahan Ciparanje Universitas Padjadjaran, Jatinangor, Sumedang. Bahan yang digunakan pada penelitian ini, yaitu F2 Ciherang x Kitaake, Ciherang, dan Kitaake. Metode penelitian yang digunakan adalah metode eksperimen tanpa rancangan tata ruang dengan uji Chi- Kuadrat untuk mendapatkan pola pewarisan dan Seleksi Rangking untuk seleksi umur singkat keluar malai pada tanaman. Hasil penelitian menunjukkan bahwa pola pewarisan karakter umur keluar malai dari F2 hasil persilangan Ciherang x Kitaake, yaitu rasio 3 : 1 diduga dikontrol oleh monogenik dengan aksi gen dominan sempurna. Tiga genotip terseleksi, yaitu genotip 115, 135, dan 195 memiliki umur keluar malai yang singkat dari persilangan Ciherang x Kitaake. Pola pewarisan tersebut diwariskan secara kualitatif dan dikendalikan secara simple genic.