Construction of Binary Vector and Transformation of Synthetic LcCsp Gene into Nipponbare Rice Genome by Agrobacterium tumefaciens Transformation Method

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dc.contributoren-US
dc.creatorYulita, Dina Sri; Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Nusa Bangsa.
dc.creatorPolosoro, Aqwin; Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development (ICABIOGRD)
dc.creatorSisharmini, Atmitri; Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development (ICABIOGRD)
dc.creatorApriana, Aniversari; Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development (ICABIOGRD)
dc.creatorNurilmala, Febi; Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Nusa Bangsa.
dc.creatorTrijatmiko, Kurniawan Rudi; Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development (ICABIOGRD)
dc.date2020-07-17
dc.date.accessioned2021-09-07T02:50:10Z
dc.date.available2021-09-07T02:50:10Z
dc.descriptionCold shock protein (Csp) is an essential bacterial protein for increasing abiotic stress tolerance, especially cold stress. Several studies discovered that overexpression of the gene successfully improves the tolerances of several types of plant not only under cold stress, but also other abiotic stresses, e.g. hot and drought conditions. The objectives of this study were to construct a binary vector containing the LcCsp gene modified from Lactobacillus casei and transform the gene into Nipponbare rice genome. The native LcCsp gene sequence, however, has low GC content (46.7%) while rice as transformation target plant has 52% GC content. The native LcCsp gene sequence, therefore, was optimized to the level close to 52.7% similar to GC content of the rice genome. This LcCsp gene was synthesized by using DNA printing technology (gBlocks® Gene Fragments Entry, IDT). The synthetic LcCsp gene was successfully inserted into the pCAMBIA1300-int binary vector driven by Ubiquitin1 promoter and NOS terminator. The T-DNA cassette was successfully transformed into Nipponbare rice genome by Agrobacterium tumefaciens strain LBA4404 using immature embryo transformation protocol. A total of 51 T0 Nipponbare lines survived from hygromycin selections and 21 lines were successfully acclimatized. Molecular analysis of the candidate lines showed that all Nipponbare transgenic putative lines contain the LcCsp gene demonstrating high transformation efficiency of 11.8%. The rice lines resulted from this study should be further analyzed and might be useful for developing rice transgenic lines tolerance to heat, drought, or saline stress condition.en-US
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dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/11047
dc.identifier10.21082/jbio.v16n1.2020.p25-34
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/13357
dc.languageeng
dc.publisherBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanianen-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/11047/9182
dc.rightsCopyright (c) 2020 Jurnal AgroBiogenen-US
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0en-US
dc.sourceJurnal AgroBiogen; Vol 16, No 1 (2020): June; 25-34en-US
dc.source2549-1547
dc.source1907-1094
dc.subjectRice; LcCsp gene; abiotic stresses; Lactobacillus casei; transformationen-US
dc.titleConstruction of Binary Vector and Transformation of Synthetic LcCsp Gene into Nipponbare Rice Genome by Agrobacterium tumefaciens Transformation Methoden-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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