Response of Five Kenaf Accessions to Shoot Regeneration

Simple item page
dc.contributor.authorPurwati, Rully Dyah
dc.contributor.authorSudjindro
dc.contributor.authorSudarmadji
dc.contributor.otherBalai Penelitian Tanaman Tembakau dan Seraten_US
dc.date.accessioned2022-04-11T03:41:35Z
dc.date.available2022-04-11T03:41:35Z
dc.date.issued1999
dc.description.abstractThis experiment was aimed to study response of fives kenfa accession to shoot regeneratioo and to establish an appropriate regeaeraiea protocol for kenaf The experiment was performed at Tissue Culture Laboratory Research Institute for Tobacco and Fibre Crops (RITFC), MaJang, from June to Octo- ber 1997. Crtyledons with plumules attached were used as explants and culture medium for callus induction was MS-based medium with BAP (2 mgfl) and NAA (0.5 mgfl). Calli produced in this cultures were transferred into MS-based medium containing BAP (2 mgfl) and GAJ (5 mgfl) for shoot initiatioo. All shorts obtained were then sub-cultured in MS-based medium without regulators (MSO) for root formation. Rooted shorts (plantlets) were acclimatized in the sterile sand and transferred into sterile soil in the glass house. Results of this experiment showed that the most respoosive accessions were Cuba 10811, followed by KK 60, He 48, PI 324922, aid CRNI 056 H with the average number of shoots per explant 4.32 ± 4.21, 4.00± 4.01, 3.05 ± 2.98, 2.80± 1.47, and 2.72 ± 2.49, respectively, at 40 days after transferring on shoot regeneratica medium. Shoots rooted after 14 days on MSO medium with frequencies of 81.50-93.30%. Healthy plaatlets survived and grew well in soil media in the glass house. Therefore, an appropriate short regeneration protocol for kenafwas found,en_US
dc.identifier.issn0853-8212
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/15538
dc.language.isoen_USen_US
dc.publisherPusat Penelitian dan Pengembangan Perkebunanen_US
dc.subjectHibiscus cannabinus L.,en_US
dc.subjectshoots regeneration,en_US
dc.subjecttissue cultureen_US
dc.titleResponse of Five Kenaf Accessions to Shoot Regenerationen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Response of Five Kenaf Accessions to Shoot Regeneration .pdf
Size:
5.25 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Budidaya Perkebunan