Identifikasi Perubahan Karakter Agronomis Padi Transgenik Penanda Aktivasi cv. Asemandi Generasi T1

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dc.contributoren-US
dc.creatorSisharmini, Atmitri; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorApriana, Aniversari; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorNurmaliki, Diah; Mahasiswa Fakultas Pertanian, Universitas Jenderal Sudirman, Jl. Dr. Soeparno, Purwokerto 53123 JawaTengah
dc.creatorSantoso, Tri Joko; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorTrijatmiko, Kurniawan R.; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.date2016-08-23
dc.date.accessioned2019-10-09T09:40:38Z
dc.date.available2019-10-09T09:40:38Z
dc.descriptionThe activation-tagged populations of transgenic rice cv.Asemandi have been developed by introducing construct ofactivation taq Ac/Ds into rice genom of cv. Asemandi. The T1transgenic rice populations cv. Asemandi containingconstruct of activation taq have been obtained and neededto be characterized. This study aimed to identify the Bastaherbicide resistant plants, transgenic plants containing hptand bar genes, and changes in agronomic traits. Bastaresistant plant was identified by treating leaf with bastasolution. Hpt and bar genes were detected by PCR usingspecific primers. Phenotype characters were identified byobserving and measuring their agronomic parameters. Thestudy results showed that out of 315 rice transgenic cv.Asemandi T1 treated with Basta solution, 176 (55.87%) plantswere indicated to be resistant to Basta. The results of PCRanalysis revealed that eight rice transgenic cv. Asemanditested contained both hpt and bar genes. In general,compared to the nontransgenic plants, there were changesin several agronomic parameters of T1 transgenic plants cv.Asemandi, including plant height, days to flowering, days toharvesting, periods of grain filling, and weight of 100 grains.Correlation analysis showed that there was no correlationbetween days of harvesting to weight of 100 grains intransgenic rice, but there was correlation in nontransgenicrice. Transgenic rice plants cv. Asemandi with changes inthe agronomic characters will be useful for further study,such as to analyze the function of the genes.en-US
dc.formatapplication/pdf
dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/4124
dc.identifier10.21082/jbio.v9n3.2013.p107-116
dc.identifier.urihttp://124.81.126.59/handle/123456789/7727
dc.languageeng
dc.publisherBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanianen-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/4124/3440
dc.rightsCopyright (c) 2016 Jurnal AgroBiogenen-US
dc.sourceJurnal AgroBiogen; Vol 9, No 3 (2013): Desember; 107-116en-US
dc.source2549-1547
dc.source1907-1094
dc.subjectRice; transgenic; activation tagging; gene identification.en-US
dc.titleIdentifikasi Perubahan Karakter Agronomis Padi Transgenik Penanda Aktivasi cv. Asemandi Generasi T1en-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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