Multiplikasi Tunas dan Induksi Perakaran pada Ubi Kelapa (Dioscorea alata L.) dan Gembili (Dioscorea esculenta L.) Secara In Vitro

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dc.contributoren-US
dc.creatorHutami, Sri; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorPurnamaningsih, Ragapadmi; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorMariska, Ika; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorDiantina, Surya; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.date2016-08-23
dc.date.accessioned2018-05-02T06:15:02Z
dc.date.available2018-05-02T06:15:02Z
dc.date.issued2016-08-23
dc.descriptionDioscorea sp. (yam) is one of the minor tuber crops whichgrows wildly in the forest and only a few of its species arecultivated and used as main or secondary food.Conservation is needed to preserve plant genetic material.The objective of this research was to obtain methods ofplantlets propagation of D. alata L. and D. esculenta L.through in vitro culture. The research was conducted atTissue Culture Laboratory of ICABIOGRAD in 2012. Theresearch consisted of three stages. First, shoot emergence.In this experiment, young shoots were planted in MS basicmedium combined with benzyl adenine (BA) (0, 1, 3, and 5mg/l) and gibberelic acid (GA) (0 and 5 mg/l). Second, shootmultiplication. Shoots of Dioscorea which were planted inthe best medium of the first experiment were subcultured inMS medium combined with thidiazuron (0, 0.1, 0.5, 1, 2, and3 mg/l). Third, root initiation. Shoots of Dioscorea whichwere planted in the best medium of the second experimentwere subcultured in MS medium (½ MS and 1 MS)combined with indole-3-butyric acid (IBA) (0, 1, 3, and 5mg/l). Result of these experiments showed that shootemergence of D. alata L. and D. esculenta L. began at 2weeks after planting in MS medium. More plantlets of D.alata L. and D. esculenta L. were obtained by shootmultiplication in MS media. Root initiation of the Dioscoreabegan at 4 weeks after planting in MS media. The addition ofIBA (3–5 mg/l) on D. esculenta L. could not stimulate rootingbut led to the formation of callus at the base of the stembuds.en-US
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dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/4140
dc.identifier10.21082/jbio.v10n2.2014.p53-60
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/485
dc.languageeng
dc.publisherBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanianen-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/4140/3460
dc.rightsCopyright (c) 2016 Jurnal AgroBiogenen-US
dc.source2549-1547
dc.source1907-1094
dc.sourceJurnal AgroBiogen; Vol 10, No 2 (2014): Agustus; 53-60en-US
dc.titleMultiplikasi Tunas dan Induksi Perakaran pada Ubi Kelapa (Dioscorea alata L.) dan Gembili (Dioscorea esculenta L.) Secara In Vitroen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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