Purification Method and Storage of Primordial Germ Cells-Circulation for Preservation of Local Chicken

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dc.contributoren-US
dc.creatorKostaman, Tatan
dc.date2014-12-01
dc.date.accessioned2018-06-04T07:49:21Z
dc.date.available2018-06-04T07:49:21Z
dc.descriptionPrimordial germ cells (PGCs) can be used for producing transgenic chickens and preserving genetic material of avian species. Primordial germ cells are precursor of germline cells that could be made proliferation and differentiation to become spermatogonia in testes or oogonia in ovary. Primordial germ cells has a unique migration path, so that the PGCs can be isolated and collected from embryos and propagated and developed through the culture. This paper describes the isolation, collection, purification, storage and transfer of PGCs-circulation of local chickens in Indonesia. Some amount of PGCs-circulation were collected from Gaok chicken at stage 15 as the safe level using cryoprotectant DMSO and some chicks hatched after transfering the PGCs-circulation to recipient embryo. Thus, this technology would be useful for preservation of Indonesian local chicken. Key words: Local chicken, primordial germ cells, cryopreservationen-US
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dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/wartazoa/article/view/1087
dc.identifier10.14334/wartazoa.v24i4.1087
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/4682
dc.languageeng
dc.publisherIndonesian Center for Animal Research and Developmenten-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/wartazoa/article/view/1087/1093
dc.source2354-6832
dc.source0216-6461
dc.sourceWARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences; Vol 24, No 4 (2014): DECEMBER 2014; 161-170en-US
dc.titlePurification Method and Storage of Primordial Germ Cells-Circulation for Preservation of Local Chickenen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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