Aplikasi Thidiazuron secara In Vitro terhadap Multiplikasi Tunas Gambir (Uncaria gambir (Hunter) Roxb)
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Date
2021-09-15
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KOMISI NASIONAL SUMBER DAYA GENETIK
Abstract
Gambir is one of the export commodities from West Sumatra which has many benefits. This study aimed to obtain the optimum concentration of Thidiazuron (TDZ) on shoot multiplication of gambir through in vitro. The research was conducted in August-October 2020 at the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University. Experiments were prepared using a Completely Randomized Design (CRD) consisting of 5 levels of TDZ treatments and repeated 6 times. Explant in the form of gambir nodes from seed germination through in vitro. The observed data were analyzed with the F-Test at the 5% real level and followed by the Duncan New Multiple Range Test (DNMRT) at the 5% level. The result showed that all TDZ concentrations could produce compound shoots. The 0,40 ppm treatment of TDZ was the best concentration in the observation variabels of the number of shoots per explant, number of leaves per explant, and shoot height in the multiplication of gambir’s shoot.
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Keywords
Gambier, multiplication, nodes, sitokinin