Optimasi Fermentasi Nira Sorgum untuk Produksi Etanol dengan Menggunakan Isolat Yeast Saccharomyces cerevisiae DBY-1
No Thumbnail Available
Date
2021-09-15
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
KOMISI NASIONAL SUMBER DAYA GENETIK
Abstract
Bioethanol is environmental friendly renewable energy and can be used as a substitute for fuel oil. Fermentation of plant material containing glucose using yeast is usually used for the production of bioethanol. The raw material of sorghum juice can be used for bioethanol production because it contains approximately of 15-21% glucose, 70% sucrose and 10% fructose. The objective of this study was to determine the optimal conditions for the fermentation process of sorghum juice with using yeast isolate Saccharomyces cerevisiae DBY-1. Several treatments were given in this study, e.g the effect of pH, sterilization, incubation conditions, the addition of nitrogen and amount of inoculants. The parameters observed in this study were ethanol content, substrates utilization and the resulting by-products. The results of this study indicated that the optimal condition were the media sterilized using autoclave, the pH is 3.7, incubated in a static incubator, the addition of urea as a nitrogen source of 2 g/L and the addition of 6 g cells/L. Through this conditions can be obtained the highest ethanol content 4.24% and density of microorganisms by 5.62. Based on the results of this study, the optimal conditions in the fermentation process to produce ethanol were influenced by the sterility of the fermentation media, pH, place of incubation, the addition of urea as a nitrogen source and amount of inoculants.
Description
Keywords
Bioethanol, Saccharomyces cerevisiae DBY-1, sorghum juice