Analisis Molekuler Gen Partenokarpi DefH9-RI-iaaM pada Progeni Tomat Transgenik

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dc.contributoren-US
dc.creatorPardal, Saptowo J; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorSlamet, Slamet; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorPurnamaningsih, Ragapadmi; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorLestari, Endang G.; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorSutini, Sutini; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia Telp. (0251) 8337975; Faks. (0251) 8338820
dc.date2016-08-09
dc.date.accessioned2019-10-09T09:40:27Z
dc.date.available2019-10-09T09:40:27Z
dc.descriptionThe development of seedless tomato fruits will be more attractive to both consumers and industries. Seedless tomatoes can beproduced through parthenocarpy technology. Artificial parthenocarpy can be induced by conventional crossing, hormoneapplication, or genetic engineering. The development of parthenocarpic tomatoes through genetic engineering has been carriedout by inserting DefH9-iaaM parthenocarpic geneinto tomato genome via Agrobacterium tumefaciens mediated transformation.Sixty putative transgenic tomato lines were produced and three events (OvR1#14-4, OvM2#10-1, and OvM2#6-2) were selectedas the best events. The background of the tomato lines was Oval variety, and based on PCR results, the three selected linescontained DefH9-RI-iaaM in their genome. The objective of this research was to determine the integration of DefH9-RI-iaaMgene in the progenies of three transgenic tomatoes lines using PCR technique. The research was conducted in the laboratoryand Biosafety Containment Facility of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research andDevelopment (ICABIOGRAD). Parental variety, Oval (neither transgenic nor in vitro cultured), and elite line of CL 6046 were usedas control plants. The results indicated that the progenies (T1, T2, and T3) of the three tomato lines contained the insert DefH9-RIiaaMgene.en-US
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dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3821
dc.identifier10.21082/jbio.v11n1.2015.p33-40
dc.identifier.urihttp://124.81.126.59/handle/123456789/7680
dc.languageeng
dc.publisherBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanianen-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3821/3170
dc.rightsCopyright (c) 2016 Jurnal AgroBiogenen-US
dc.sourceJurnal AgroBiogen; Vol 11, No 1 (2015): April; 33-40en-US
dc.source2549-1547
dc.source1907-1094
dc.subjectMolecular analysis; transgenic tomato lines; parthenocarpic gene; genetic engineering.en-US
dc.titleAnalisis Molekuler Gen Partenokarpi DefH9-RI-iaaM pada Progeni Tomat Transgeniken-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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