Keragaman Genetik Strain Ralstonia solanacearum berdasarkan Karakterisasi Menggunakan Teknik Berbasis Asam Nukleat
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Date
2002
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Publisher
Balai Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian
Abstract
Genetic Variability of Ralstonia solanacearum Strain based on the Characterization
using DNA Analysis Technique. Yadi Suryadi and M. Machmud. Ralstonia solanacearum
the causal agent of bacterial wilt disease has a wide range of genetic variability. Nucleic acidbased analysis to characterize the pathogen i.e. Restriction Fragment Length Polymorphism (RFLP) technique has shown two different groups of strains which corresponded with geographical origins. The DNA fingerprint analysis developed recently Included screening DNA primer of various PCR-based assay against genomic DNA of R. solanacearum. Studies on the characteristics of genomic DNA of R. solanacearum isolates have been done i.e. using Random Amplified Polymorphic DNA (RAPD) and Repetitive Extragenic Palindromic (REP) analysis. These diagnosis assays will be of further used to provide specific DNA profiles that can be applied for epidemiological study and bacterial wilt control as well.
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Keywords
R. solanacearum, genetic variability, diagnosis