Pemanfaatan Penanda SSR untuk Analisis Sidik Jari DNA Kentang (Solanum tuberosum L.)
| dc.contributor.author | Rizkyantoro ...[at al], Ahmad Fadil | |
| dc.contributor.other | Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian | en_US |
| dc.date.accessioned | 2021-12-23T03:06:03Z | |
| dc.date.available | 2021-12-23T03:06:03Z | |
| dc.date.issued | 2021-09-15 | |
| dc.description.abstract | DNA fingerprinting analysis in plant breeding program is required due to the confirmation of genetic identity on a variety, variety registration, and plant variety protection (PVP). Establishment of DNA fingerprinting was conducted on 5 variety candidates of potato POR 19028, POR 19053, POR 20059, POR 20061, POR 20073 with 17 other comparison potatoes using 25 SSR markers with fluorescently labeled on a DNA analyzer based on the capillary electrophoresis. The technology facilitates DNA allele sizes reading precisely and accurately thus the genetic diversity among the variety can be further identified. A total of 80 SSR alleles were obtained at 25 SSR loci with a total of 1-9 alleles per locus. Unique SSR alleles can be used as genetic markers from each variety. The results of allele comparison in the entire SSR loci were obtained 7 unique SSR markers for 5 potato variety candidates, including STM0001, STM0003, STM0019a, STM1025, STM1106, STM2013, and STM3012. The genetic diversity analysis using a NTSYS ver 2.2 software was produced groups, consisting of POR 20059, POR 20061, and POR 20073 which had genetic similarity 71-73% to the other types of processed potatoes. POR 19053 had 78-80% genetic similarity to the vegetable and intermediate potatoes and POR 19028 to the other vegetable potatoes was 90-95%. | en_US |
| dc.identifier.isbn | 978-9798-3930-7-5 | |
| dc.identifier.uri | https://repository.pertanian.go.id/handle/123456789/14455 | |
| dc.language.iso | id | en_US |
| dc.publisher | KOMISI NASIONAL SUMBER DAYA GENETIK | en_US |
| dc.subject | DNA fingerprinting, potato, SSR, unique markers | en_US |
| dc.title | Pemanfaatan Penanda SSR untuk Analisis Sidik Jari DNA Kentang (Solanum tuberosum L.) | en_US |
| dc.type | Book | en_US |