Teknik Isolasi dan Kultur Protoplas Tanaman Padi

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dc.contributoren-US
dc.creatorSukmadjaja, Deden; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111
dc.creatorSunarlim, Novianti; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111
dc.creatorLestari, Endang G.; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111
dc.creatorRoostika, Ika; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111
dc.creatorSuharlini, Tintin; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111
dc.date2016-08-09
dc.date.accessioned2021-09-07T02:50:16Z
dc.date.available2021-09-07T02:50:16Z
dc.descriptionProtoplastfusion or somatic hybridization technology is an alternativetechnology for production hybrids of plants that are difficultto be produced by conventional methods due to their sexualincompatibility. An experiment was conducted to developtechniques for isolation, purification, and culture of riceprotoplasts of cultivar IR64 and a wild rice species (Oryzaofficinalis). Optimization of protoplast isolation and purificationmethods from both rice genotypes were successfullydone. The highest protoplast density was obtained bydigesting embryonic callus or stems of young seedling in anenzyme solution containing of 2% cellulose, 0.1% pectolyase,0.5% macerozyme, 0.5% driselase, 5 mM ES, and 13% mannitolin CPW solution. The protoplast digestion was done forthree hours by soaking in the enzyme solution followed byshaking at 50 rpm under a room temperature. Purification ofthe protoplasts were done by separating them from plantdebris using a 25% sucrose solution. Protoplast regenerationwas not successful using although different media compositionsand conditions. Growth process from cell division tocell aggregate was only successful on IR64 protoplast cultureon a medium that contained AgNO3.en-US
dc.formatapplication/pdf
dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3781
dc.identifier10.21082/jbio.v3n2.2007.p60-65
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/13363
dc.languageeng
dc.publisherBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanianen-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3781/3130
dc.rightsCopyright (c) 2016 Jurnal AgroBiogenen-US
dc.sourceJurnal AgroBiogen; Vol 3, No 2 (2007): Oktober; 60-65en-US
dc.source2549-1547
dc.source1907-1094
dc.subjectProtoplasts; isolation and culture; cultivated and wild rices.en-US
dc.titleTeknik Isolasi dan Kultur Protoplas Tanaman Padien-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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