Inheritance of Resistance Gene to Cucumber Mosaic Virus in Maize
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Date
2013
Journal Title
Journal ISSN
Volume Title
Publisher
Badan Penelitian dan Pengembangan Pertanian
Abstract
Maize (Zea mays L.) is one of important crop in the world. In Indonesian, maize production has been
increasing, due to high consumption. Madura island is the largest areal for maize production in East Java and
Indonesia. The island has also some local maize. Virus infection, including Cucumber Mosaic virus (CMV) could
reduced maize production until 62.2%. Hence, it is important to improve the quality of Madura’s local maize by
resistant breeding. The aim of this research was to study on the inheritance of resistance gene to CMV in crosses
between Guluk-guluk, a Madura’s local cultivar, and Srikandi Kuning 1. Guluk-guluk was provided by Assessment
Institute for Agricultural Technology Malang, East Java Indonesia, while maize Srikandi Kuning 1 was provided by
Indonesian Cereals Research Institute Maros, South Sulawesi. Resiprocal breeding experiments were conducted
between Guluk-guluk and Srikandi to obtain F1 population. To produce F2 population, F1 individuals were selfed. On the
other hand, other F1 individuals were crossed to Srikandi Kuning 1 to produce testcross population. The resistance
to CMV in maize population was evaluated using symptoms observation and Enzyme-linked Immunosorbent Assay
(ELISA) analysis. Furthermore, the data of resistance to CMV in maize was analyzed using chi-square. Results of
this research showed that Guluk-guluk exhibits resistance to CMV, while Srikandi Kuning 1 was susceptible. All F1
plants showed resistance to CMV, while F2 and testcross populations showed segregation with ratio 3 : 1 (F2) and
1 : 1 (testcross). Seventy two individuals of progeny (F2) showed resistances and 27 were susceptible. Ten
individuals of testcross are resistance, while 9 plants are susceptible. Based on chi-square analysis, it indicates
that F2 (72:27) and testcross (10:9) demonstrated Mendel’s law. The results of the study revealed that the inheritance
of resistance gene to CMV in crossing between Guluk-guluk and Srikandi Kuning 1 is controlled by a single dominant
gene Zrec.