Perkembangan Penelitian Regenerasi dan Transformasi pada Tanaman Kedelai
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Date
2002
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Balai Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian
Abstract
Progress of Plant Regeneration and Transformation on Soybean. Saptowo J. Pardal.
Soybean Is one of the genotype that still difficult to manipulate in vitro. Some reports showed that soybean can be regenerated through in vitro culture, but infact it can not be repeated successfully by other laboratories. Type of cells or explants and genotype play important role in the plant regeneration of soybean. Soybean mainly can be regenerate through two pathways, i.e. shoot morphogenesis (organogenesis) and somatic embryogenesis. Both pathway have an advantage and disadvantage. Shoot morphogenesis can be induce easily from the cotyledonary node segment of soybean and it will have the same character as a parent, but the number of shoot is limited. On another hand, somatic embryogenesis can not be induce easily from the soybean explants, but the number of embryos or embryogenic callus is plenty if it can
be induced. Due to the difficulties of plant regeneration of soybean so far, the genetic
transformation of soybean is still far from the routine system. Although it has been reported by the two laboratories using two different protocols. The success of soybean transformation with foreign gene is reported in 1988 by using Agrobacterium tumefaciens and particle bombardment method.
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Keywords
Soybean, plant regeneration, genetic transformation