Deteksi Produk Rekayasa Genetika: Blind Test untuk Sampel Campuran Tepung
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Date
2021-09-15
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KOMISI NASIONAL SUMBER DAYA GENETIK
Abstract
Regulations related to the biosafety of genetically modified products (GMO) are contained in Government Regulation (PP) No. 21 of 2005. Nowadays, 27 products have obtained food safety certificates. With the implementation of these regulations, it is necessary to have a laboratory that can detect GMOs. This research aimed to test the capability of BB Biogen as a GMO detection laboratory in determining the origin of the event and the flour samples sent by Jabatan Kimia Malaysia for the proficiency test. The method used was qualitative PCR using primers and positive controls available at BB Biogen. The results of this test indicate that the flour samples contain soybean and corn plant elements. In addition, the flour has a 35S promoter and a NOS terminator, while MON87988 successfully amplified the target DNA by actual size, indicating that the flour samples tested contained RSS2 (MON89788) soybeans and probably RSS soybeans and/or NK603 maize. The Molecular Biology Laboratory of BB Biogen is capable of detecting the content of GMO material in Blind Test samples.
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Keywords
GMO, food safety, PCR