Browsing by Author "Yuliani ...[at al], Dini"

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    Ketahanan Galur Isogenik IRBBN dan Galur Harapan Padi terhadap Patotipe Xanthomonas oryzae pv. oryzae Dominan pada Tanaman Padi di Indonesia
    (Sekretariat Komisi Nasional Plasma Nutfah, 2017) Yuliani ...[at al], Dini; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    Galur isogenik IRBB introduksi dari International Rice Research Institute (IRRI) telah diketahui gen ketahanannya dan dapat digunakan sebagai sumber ketahanan terhadap patotipe Xanthomonas oryzae pv. oryzae (Xoo) dominan di Indonesia. Kegiatan penelitian ini bertujuan untuk mengevaluasi ketahanan galur isogenik dan galur harapan terhadap patotipe Xoo dominan di Indonesia, yaitu patotipe III, IV, dan VIII. Penelitian dilaksanakan di Kebun Percobaan Sukamandi, Subang, Jawa Barat pada musim kemarau (MK) 2012 dan musim hujan (MH) 2012/2013. Penelitian dilakukan dengan Rancangan Faktorial Acak Kelompok dengan tiga ulangan. Faktor pertama adalah tiga patotipe Xoo, yaitu patotipe III, IV, dan VIII, sedangkan faktor kedua yaitu materi yang diuji sebanyak 20 galur IRBB, 6 galur harapan padi, dan 6 varietas pembanding, yaitu Conde, Lusi, Logawa, Java 14, Angke, dan Inpari 1. Hasil penelitian menunjukkan bahwa galur isogenik IRBB 21, IRBB 50, dan IRBB 52 bereaksi tahan terhadap Xoo patotipe III, IV, dan VIII pada MK 2012. Pada MH 2012/2013 diperoleh lima galur isogenik IRBB 52, IRBB 53, IRBB 54, IRBB 56, dan IRBB 57 bereaksi tahan terhadap Xoo patotipe III, IV, dan VIII. Galur isogenik IRBB 52 konsisten tahan terhadap Xoo patotipe III, IV, dan VIII pada dua musim tanam. Oleh karena itu, galur tersebut dapat direkomendasikan untuk dijadikan tetua tahan untuk perakitan varietas tahan HDB.
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    Selection Resistance of Rice Germplasm Accessions to Bacterial Leaf Blight
    (Sekretariat Komisi Nasional Plasma Nutfah, 2014) Yuliani ...[at al], Dini; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    Rice cultivation in Indonesia has been faced with many obstacles, including the attack of bacterial leaf blight (BLB) that caused by bacteria Xanthomonas oryzae pv. oryzae (Xoo). Resistant variety was considered as the cheap technique to control BLB disease and could be used by rice farmers. Germplasm accessions as a source to build resistant varieties must be known their resistance to BLB disease. This study aimed at selecting the resistance germplasm accessions to BLB pathotype III, IV, and VIII. The study was conducted at the Experimental Station of the Indonesian Center for Rice Research in Sukamandi, Subang, West Java at wet season 2012/2013 and dry season 2013 with Split Plot Design. The main plot was three pathotypes Xoo i.e. pathotype III, IV, and VIII. The subplot was 20 germplasm accessions and three check varieties. A total of 20 hills of rice plants per plot were inoculated by Xoo with cutting method. Inoculation was conducted before the primordia stage. Inoculation of bacterial suspension containing Xoo aged 48 hours at a concentration of 108 cfu. Observations BLB disease severity was done by measuring the longest length of symptoms at the age of two, three, and four weeks after inoculation (WAI). The result showed that three germplasm accessions from near isogenic lines were not significantly different with the check varieties (Angke) in their resistance to Xoo pathotype III, IV, and VIII in two cropping seasons. The isogenic lines were IRBB 55, IRBB 60, and IRBB 61 can be used in the assembly of resistant new varieties to BLB.