Browsing by Author "Terryana ...[at al], Rerenstradika Tizar"

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Identifikasi 27 Varietas Cabai Menggunakan Beberapa Jenis Marka Molekuler dan Asosiasinya dengan Ketahanan Antraknosa
    (KOMISI NASIONAL SUMBER DAYA GENETIK, 2021-09-15) Terryana ...[at al], Rerenstradika Tizar; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    Chilli is a strategic horticultural commodity in Indonesia with high economic value and needs improvement for anthracnose resistance. Molecular markers availability has increased the development of DNA fingerprinting technology for various objectives, i.e. genetic diversity, varietal identification, and assisting selection stage in plant breeding. This study aimed to identify commercial varieties of chilli in Indonesia using several types of PCR-based molecular markers and their association with anthracnose resistance. A total of 27 chilli varieties consisting of into two species groups (Capsicum annuum L. and C. frutescent L.) were genotyped using 12 molecular markers (8 SNAP (single nucleotide amplified polymorphisms), 2 RAPD (random amplified polymorphic DNA), 1 INDEL (insertions-deletions), and 1 SSR (simple sequence repeats)). Varietal resistance to anthracnose was also tested in vitro using Colletotrichum capsici isolate. The results showed an average polymorphism information content (PIC) value of 0.24 (0.07-0.71). Based on phylogenetic analysis, 27 chilli varieties were divided into two main groups with different genetic similarity coefficients depending on the type of molecular markers. Three sets of molecular markers, which are 8 SNAP markers, a combination of 4 markers (2 RAPD, 1 INDEL, and 1 SSR), and all of the molecular markers used, can clearly distinguish chilli varieties but at different cut points, 0.78, 0, 60 and 0.67, respectively. The combined 4 types of molecular markers showed the highest genetic differentiation value for chilli varieties used. Importantly, OPE18 was significantly associated with resistance to anthracnose, which is promising to assist the selection process in chilli breeding after further assay.
  • No Thumbnail Available
    Item
    Morphological, Physiological, and Molecular Identification and Characterization of Yeast Isolated from Indonesian Fruits and Woods
    (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, 2022-01-19) Terryana ...[at al], Rerenstradika Tizar; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
    Abstract. Yeast is one of important microorganisms in the biofuel industry in recent years. Identification and characterization of yeast are important for determining the quality and purity of yeast strains. This study intended to characterize yeast isolated from persimmon, special woods from Indonesia, and commercial yeast as a comparison. Macroscopic and microscopic morphological observation was performed to preliminarily identify the species of yeast isolates, supported with molecular characterization based on ITS fragment. The physiological aspect of yeast was assessed on their sensitivity to ethanol and a comparative experiment with glucose and sucrose substrate. A total of seven isolates were obtained. All yeast isolates had milky white color and slimy consistency, except for YK1.3 and YBK1, which had a watery consistency. Several isolates have oval and round shapes with blue color. All isolates were successfully sequenced based on the internal transcribed spacer (ITS) 1 and ITS4 regions. Homology analysis of the sequences against the GenBank database using BLASTN showed that seven isolates have homology with the Debaryomyces hansenii, Wickerhamomyces anomalus, Clavispora lusitaniae, and Metschnikowia agaves species. Physiologically, YRt2.1, YK1.3, YK2.2, and YK3.2 belonging to D. hansenii, C. lusitaniae, M. agaves, and W. anomalus, respectively, had better growth on glucose substrate incubated for 24 h. In contrast, YRt1.2, YB3.1, and YBK1 belonging to D. hansenii, W. anomalus, and D. hansenii, respectively, grew well on sucrose rather than glucose substrate. All isolates with glucose substrate seemed to be more tolerant to ethanol. Moreover, isolates YRT1.1 produced the highest ethanol concentration level until 39.28 ppm compared to the other isolates. This study clearly illustrates that identification based morphological and physiological characters as well as ITS fragment could be useful taxonomic tools for rapid identification at the species level of unknown isolated strains and constitutes a basis for initial yeast strain selection for future application in the production of ethanol.