Browsing by Author "Sukmadjaja ..[at al], Deden"

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    Characterization of Japansche Citroen Rootstock Somaclones and In Vitro Selection for Aluminium Tolerance
    (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, 2022-01-19) Sukmadjaja ..[at al], Deden; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
    Abstract. Japansche citroen (JC) is a type of citrus which is widely used as rootstock in citrus cultivation. In order to expand citrus cropping on marginal acid land, a JC citrus clones that are tolerant of acid soil conditions are needed. Therefore, it is necessary to assemble the citrus genotype by increasing genetic diversity to obtain tolerant traits for aluminum and low pH. In this study, mutation induction experiments with gamma ray irradiation were carried out, followed by in vitro selection methods. The aims of this research was to increase genetic variation of JC citrus through induced mutation by gamma ray irradiation and to obtain candidates of JC citrus somaclones that are tolerant to Al at low pH. The research was conducted at the Tissue Culture Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Bogor. The plant material used was JC citrus plantlet from immature embryos treated with gamma ray irradiation at doses of 0, 4.5, 5, and 5.5 Gy. Evaluation was conducted to morphological characters, followed by in vitro selection for tolerance to Al at low pH by using selecting agent of aluminium trichloride hexahydrate. The results showed that morphological variations found in JC citrus somaclones after being irradiated with gamma rays, including the formation of the winged leaves (unifoliolatus) that are not found in the control JC. There were 32 selected somaclones to be tolerant to Al at low pH.