Browsing by Author "Sigit Budi Santoso"

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    Microsatellite-Based Markers Selection to Improve Hybrid Maize Breeding
    (Badan Penelitian dan Pengembangan Pertanian, 2013) Marcia B. Pabendon; Sigit Budi Santoso; Nuning Argo Subekti
    Information about genetic variability is fundamental in the development of hybrid varieties. Conventional breeding technologies are now supported by molecular tools to enhance the efficiency of hybrid breeding program. Microsatellite markers, for example, is able to detect co-dominant locus which is suitable to use in open pollinated crop such as maize. Results of characterization on a set of elite inbred lines via microsatellite-based markers at ICERI (Indonesian Cereals Research Institute) Molecular Biology, offers essential data for current works in hybrid breeding program. The Information on the level of homozygosity of eight sets of advanced inbred suggests that the level of homozygosity is varied from 2.6-40.0%. This means that a careful selection is required in deciding suitable inbred lines for further phase in hybrid breeding. The genetic diversity of the eight sets of elite inbred showed that there were four sets of inbred which have low degree of polymorphism. This indicated that genetic variability among the inbreds were narrow, where crosses between inbred of the same set should be avoided. Another result was the merging of two sets of elite inbred that own high yielding potential and acid soil tolerant, which provided information on the possibility of heterotic pairs available based on genetic distance values > 0.7. It was also found that, based on several research results, the use of microsatellites-based markers in the improvement of hybrid breeding programs would make the effort to be more focus and efficient.