Browsing by Author "Rizkyantoro ...[at al], Ahmad Fadil"

Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    Pemanfaatan Penanda SSR untuk Analisis Sidik Jari DNA Kentang (Solanum tuberosum L.)
    (KOMISI NASIONAL SUMBER DAYA GENETIK, 2021-09-15) Rizkyantoro ...[at al], Ahmad Fadil; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    DNA fingerprinting analysis in plant breeding program is required due to the confirmation of genetic identity on a variety, variety registration, and plant variety protection (PVP). Establishment of DNA fingerprinting was conducted on 5 variety candidates of potato POR 19028, POR 19053, POR 20059, POR 20061, POR 20073 with 17 other comparison potatoes using 25 SSR markers with fluorescently labeled on a DNA analyzer based on the capillary electrophoresis. The technology facilitates DNA allele sizes reading precisely and accurately thus the genetic diversity among the variety can be further identified. A total of 80 SSR alleles were obtained at 25 SSR loci with a total of 1-9 alleles per locus. Unique SSR alleles can be used as genetic markers from each variety. The results of allele comparison in the entire SSR loci were obtained 7 unique SSR markers for 5 potato variety candidates, including STM0001, STM0003, STM0019a, STM1025, STM1106, STM2013, and STM3012. The genetic diversity analysis using a NTSYS ver 2.2 software was produced groups, consisting of POR 20059, POR 20061, and POR 20073 which had genetic similarity 71-73% to the other types of processed potatoes. POR 19053 had 78-80% genetic similarity to the vegetable and intermediate potatoes and POR 19028 to the other vegetable potatoes was 90-95%.