Browsing by Author "Rahmawati, Syamsidah"

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    Gen Penyeleksi Alternatif untuk Transformasi Tanaman
    (Balai Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian, 2003) Rahmawati, Syamsidah; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    Alternative Selectable Marker Gene for Plant Transformation. Syamsidah Rahmawati. At the moment, antibiotic and herbicide resistant genes are the most commonly used selectable markers for plant transformation. However, issues on allergenicity or toxicity, gene transfer and others, which were suspected to cause negative impact on human health and environment, have raised debate of using this selection system around the world. Many non government organizations world wide, including the Commission of the European Communities, discourage the use of the antibiotic selection system. Based on these reasons, it is important to find alternative selectable markers. In this paper, four alternative selectidn systems that are potentially safe and reported effective in plant transformation system were discussed. Two systems, the phosphomannose isomerase (PMI) and xylose isomerase (Xyla), used mannose and xylose, respectively, as selective agents. Furthermore, enzyme xylose isomerase has been widely used in starch industry and certain food processing. MAT vector system was developed to produce selectable marker-free transgenic plants, while the green fluorescent protein (GFP) enable to select transformed tissues visually. These systems have been applied in various plant species.