Browsing by Author "Priyatno ...[at al], Tri Puji"

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    In Silico Functional Prediction of CAS2, a Protein Specifically Expressed in Appressorium and Required for Pathogenicity of Colletotrichum gloeosporioides
    (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, 2022-01-19) Priyatno ...[at al], Tri Puji; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
    Abstract. Colletotrichum appressoria specific gene (CAS2) expressed during appressorium formation of Colletotrichum gloeosporioides play important role as virulence factors. Determination of the CAS2 protein structure could help deduction of its function and interaction with other factors. The current study aimed to assess in the functional and structural properties of the CAS2 by in silico analyses. CAS2 sequence submitted to the online tool Gene Ontology Functional Enrichment Annotation Tool (GO FEAT) in order to search for homology. The biological importance of the annotated products were characterized based on the analyses of physicochemical properties, subcellular localization, molecular function, protein-protein interaction networks, and 3D structural protein modelling. The results showed that CAS2 is a hydrophobic protein with an average molecular weight of around 46.2 kDa. The N-terminal amino acid (aa) sequence of CAS2 was composed of 22 amino acid residues of signal peptide. The high score probability (1.840) of subcellular location prediction showed that this protein located in plasma membrane. Based on putative transmembrane prediction software, the CAS2 protein is composed of three alpha-helical transmembrane domains with the longest of C-terminal of amino acid residues in cytoplasm. CELLO2GO server predicted that the CAS2 is a structural protein functioning as enzyme and ion binding. Protein interaction network resolved by STRING web server revealed 10 potential interacting protein associates with CAS2. These in silico analysis offered excellent and reliable information for functional characterization of CAS2 protein by using the advanced tools and techniques of computational biology.
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    Teknik Produksi dan Formulasi Bakteri Kitinolitik untuk Pengendalian Penyakit Karat Kedelai
    (Balai Penelitian Bioteknologi Tanaman Pangan, 2001-12) Priyatno ...[at al], Tri Puji; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    Teknik produksi dan formulasi tiga isolat unggulan bakteri kitinolitik (6a, 6m, dan 7) telah dipelajari untuk pengembangan biofungisida patogen karat kedelai (Phakopsora pachyrhizi). Di antara empat jenis media yang diuji, PDYB dengan kandungan yeast extract 0,75% merupakan media yang paling efisien dan efektif menghasilkan jumlah sel bakteri tertinggi. Waktu panen sel yang terbaik adalah 24 jam setelah inkubasi. Isolat 6m dan 7 memproduksi sel tertinggi pada suhu 32oC sedangkan isolat 6a pada suhu 24oC. Kecepatan agitasi optimum untuk ketiganya adalah 125 rpm. Formulasi bakteri kitinolitik dalam bentuk cair, mengandung isolat tunggal maupun kombinasi, lebih efektif menekan perkem-bangan penyakit karat di rumah kasa dibandingkan dengan formulasi tepung. Formulasi cair mengandung kombinasi isolat 6m dan 7 bersifat sinergistik di-bandingkan dengan pengaruh formulasi masing-masing isolat dalam menekan perkembangan penyakit, yaitu sebesar 52,7%.