Browsing by Author "Pardal ...[at al], Saptowo J."

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    Backcrossing of Soybean Lines Containing Aluminium Tolerance Gene into Superior Soybean Variety, Biosoy
    (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, 2022-01-19) Pardal ...[at al], Saptowo J.; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
    Abstract. Two new superior varieties of soybeans, Biosoy 1 and Biosoy 2 have the advantage of high productivity and bigger seeds than favorite local variety Grobogan. These varieties have been planted in several provinces in Java and showed a good performance. However, these varieties are not so adaptive in acid soil with high aluminum content. Therefore, to expand the planting area of Biosoy varieties outside Java, which is generally an acidic land with high Al content, it is necessary to improve its tolerance to Al stress. Two transgenic soybean lines (GM2 and GM5) carrying the Al tolerance gene (MaMt2) have shown tolerance to acidic soils pH 3.7–4.8 based on evaluation in the Biosafety Containment. Those transgenic lines have been crossed with the Biosoy variety. F1 soybean plants carrying the MaMt2 gene were then backcrossed to the Biosoy variety to produce BC1F1 seeds. The aim of this research was to do molecular confirmation of MaMt2 gene insertion in the BC1F1 soybean plants and backcrossing the positive BC1F1 to Biosoy 1 variety. Materials used for the research were seven plants of BC1F1-GM2, six plants of BC1F1-GM5, and Biosoy 1 variety. The research works included PCR analysis of BC1F1 plants, backcrossing of BC1F1-MaMt2 plants with Biosoy 1, and harvesting of BC2F1 seeds. The PCR analysis of 13 samples of BC1F1 plant showed that all samples were positive carrying the MaMt2 gene (100%). BC2F1 seeds have been produced from the second backcross. These BC2F1 soybean seeds will be used as backcross material in the next studies.