Browsing by Author "Martasari ...[at al], Chaireni"

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    Cytological and Molecular Identifications of Seedless Tangerine Derived from Endosperm Culture
    (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, 2022-01-19) Martasari ...[at al], Chaireni; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
    Abstract. Endosperm cultures have been established on tangerine citrus cv. Siam Madu. To verify the ploidy levels of the regenerant derived-plants, both cytological and molecular assessments were applied. The research was conducted from March until June 2017 at the Laboratory of Plant Breeding and Genetic Resources of the Indonesian Citrus and Subtropical Fruits Research Institute (ICSFRI). A total of 25 tangerine citrus accessions served as the evaluated materials after they were grafted into JC rootstock for 16 weeks. The results showed that the stomatal density, length, and width were varied among the tested accessions. The lowest stomatal density was observed in SM42. While, SM11 showed the highest stomatal density with smallest value in stomatal size trait. The highest ratio of stomatal length and width was detected in SM56. Based on the cytological observation, 13 accessions were observed to have triploid, 11 accessions were diploids, and one accession had haploid chromosome structures. Molecular characterization using SSR primers (TTTA41, TAA15, and CAT01) resulted that most of tested accessions had the same number of amplified alleles, except for SM18, SM47, SM49, SM51, and SM54 which showed polymorphic bands compared to the controls.