Browsing by Author "Handharyani, Ekowati"

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    Extrahepatic bile duct ligation in broiler chickens: ultrastructural study of Ito cell
    (Indonesian Animal Sciences Society, 2014-02-21) Handharyani, Ekowati; Ochiai, K; Winarto, A
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    Natural Case Of Rabies In Stray Dogs: Pathomorphology And Antigen Distribution Studies Of Brain And Salivary Glands
    (Balai Veteriner Bukittinggi, 2016) Rahmadani, Ibnu; Handharyani, Ekowati; Harlina, Eva
    Stray dogs with positive rabies that were used in this study collected from Disease Investigation Center Bukittinggi area. The microscopic diagnose were confirmed based on direct Fluorescence Anti-body Technique (dFAT). The immunohistochemistry evaluation and distribution of virus antigen were visualized by using polyclonal antibody againt rabies. Brain tissues (hippocampus, cerebellum, cerebral cortex) and salivary gland (submandibullary and parotid gland) were collected, and fixed in 10% Buffered Neutral Formalin (BNF) then routinely processed for histopathological examination. Multifocal infiltration of lymphocytes and perivascular cuffing were midly observed in evaluated brain samples. Careful examination on hippocampus, cerebellum, and cerebal cortex showed appearance of Negry Bodies in Pyramidal and Purkinje cells. The found lesions in submandibulary glands were in severely diagnosed level, with moderate to severe infiltration of lymphocytes in acini septal and necrosis of epithel mucogenic acini cells. However, there were no significant lesions found in parotid glands. Immunohistochemical examination demonstarted that rabies virus/antigen granules were predominantly found in ammons horn of hippocampus, purkinje cells of cerebellum and pyramidal cells in cerebral cortex. Antigen were ocalized in epithel cells of mucogenic acini of submandibulary glands. In addition, antigen rabies was also observered mildly in granular and striated duct lumina of partoid glands.
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    Seroprevalence of Q fever in sheep and goat in West Java area
    (Indonesian Animal Sciences Society, 2013-06-21) Setiyono, Agus; Handharyani, Ekowati; Mahatmi, Hapsari