Browsing by Author "Andarini ...[at al], Yusi Nurmalita"

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    Evaluasi Metode Skrining untuk Cekaman Kekeringan pada Aksesi Lokal Padi Gogo
    (KOMISI NASIONAL SUMBER DAYA GENETIK, 2021-09-15) Andarini ...[at al], Yusi Nurmalita; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
    One of the potential sources of variability for assembling new rice varieties for stressful environments is local accessions that have well-adapted to a particular area. IAARD Gene Bank is one of the institutions that conserve ex-situ various types of local accessions, one of which is upland rice. To explore the genetic potential of local accessions of upland rice, an efficient and effective method for germplasm screening is needed. This study aimed to evaluate the screening method of local upland rice germplasm against drought stress. A total of nine accessions of local upland rice were evaluated for their tolerance to drought at various stress levels based on the gravimetric method. The research was conducted in the greenhouse of ICABIOGRAD Bogor during June-September 2017. The experimental was designed in a split-plot, consisted of four levels of field capacity as the main plot and nine accessions of local upland rice as sub-plots with three replications. The results showed that none of the accessions survived at 25% field capacity. Meanwhile, at 75% and 50% field capacity, the results varied among the accessions tested with higher plant survival percentages at 75% field capacity. The results obtained from this study can be used as initial useful information regarding the genetic potential of local upland rice accessions to drought stress. The screening method evaluated in this study can also be implemented as recommendations for the application of screening method for other local upland rice accessions collected the IAARD Gene Bank.
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    Genetic Relationship of Pigmented Rice (Oryza sativa L.) Collected from Eastern Indonesia Based on Morpho-Agronomical Traits and SSR Markers
    (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, 2022-01-19) Andarini ...[at al], Yusi Nurmalita; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian
    Abstract. Due to its nutritional content and beneficial health, black and red rice, which belong to pigmented rice, has become more popular. Research on pigmented rice in Indonesia has not been intensively carried out, so information on the genetic diversity of pigmented rice is very important for an effective breeding program. This research was conducted to analyze the genetic diversity of 15 accessions of local pigmented rice collected from Eastern Indonesia (Sulawesi and East Nusa Tenggara) in IAARD-ICABIOGRAD Gene Bank based on 33 morpho-agronomical characters and 24 SSR markers. The experiment was designed in a randomized complete block design with three replications. The morphoagronomical observation was performed based on a standard descriptor for rice. The morpho-agronomic characters of all observed accessions varied, indicating high diversity. Based on the characteristic of SSR markers, the average allele number, major allele frequency, gene diversity, heterozygosity, and the PIC were 4.88, 0.47, 0.66, 0.02, and 0.62, respectively. The similarity coefficient between accessions based on morpho-agronomic characters and SSR markers was still quite high (80%). PCoA and phylogenetic analyses based on the results of combined SSR and morpho-agronomic data formed three different groups at a cophenetic distance of 0.22. The result of this study is expected to provide a better understanding of the genetic characteristics of the accessions for further use to support the PGR breeding program.