Vaccination of Quails with Bivalent Inactivated H5N1 AI Vaccine (Clades 2.1.3 and 2.3.2) at Laboratory Scale
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Indonesian Center for Animal Science Research and Development (ICARD)
Abstract
Description
Quails, Coturnix sp, are commercially bred for meat and egg production in order to support the needs for animal protein. Cases of H5N1 Avian Influenza still occur sporadically at quail farms. Vaccination become an option as a precaution against possible exposure to H5N1 AI virus. Thirty quails were vaccinated with bivalent inactivated H5N1 AI vaccine (clade 2.1.3 and 2.3.2) and 10 quails were used as control group. The quails were vaccinated with one dose (0.3 ml) per bird intramuscularly at the age of 23 days and booster was done at the age of 45 days. The response after a single vaccination showed that antibody titers were not optimal, but after the booster vaccination the antibody titers showed 4.2 log2 in average against the H5N1 AI antigen of clade 2.1.3 and 3.7 log2 against the antigen of clade 2.3.2. A challenge test with H5N1 influenza virus either with clade 2.1.3 or clade 2.3.2 indicated a 70% protection. Nevertheless, viral shedding was detected ≥7 days post-challenge. As conclusion, vaccination with inactivated bivalent vaccine H5N1 AI clades 2.1.3 and 2.3.2 induced antibody that were was not homogenous nor optimal.
Keywords
Quails; H5N1 AI Vaccine; Avian Influenza